Translational_Unit

Part:BBa_J102000:Design

Designed by: Michael Pettigrew   Group: Emberly_Lab   (2010-07-02)


RBS + GFP with LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

Used GFP with LVA tag so that response of this parts (when used with a valid promoter and terminator) could be characterized more easily. Used high-efficiency, standard RBS so that there will be a larger dynamic range of GFP expression, which can be controlled at the gene level.


Source

Source is using the 3A assembly method of biobrick construction with the parts B0034 and K145015, both obtained from the 2010 iGEM DNA Distribution.

References